The Tankyrase 1 Activity Assays are high-throughput, semi-quantitative ELISAs designed to measure Tankyrase 1 activity. Tankyrase 1, also known as PARP5a, is a Poly (ADP-ribose) Polymerase (PARP) that associates with telomeres via its interaction with the telomeric-repeat binding factor TRF1. These assays utilize a histone-coated 96-well microplate and an anti-poly (ADP-ribose) (PAR) antibody to detect PAR that has been deposited onto the immobilized histone proteins by Tankyrase 1. Assays are available in a colorimetric detection format, which uses TACS-Sapphire™, or a chemiluminescent detection format, which uses PARP PeroxyGlow™ A and B. Additionally, these are the only commercially available assays to feature a full length Tankyrase 1 enzyme and unmodified NAD substrate to create a more physiologically applicable in vitro assay system.
Available Kits
- Tankyrase 1 Colorimetric Activity Assay (Catalog # 4700-096-K, 4700-192-K)
- Tankyrase 1 Chemiluminescent Activity Assay (Catalog # 4701-096-K, 4701-192-K)
Features
- Two non-radioactive detection formats available
- Amenable to high-throughput
- Highly sensitive: capable of measuring 0.1 mUnits of Tankyrase 1
- 3 hour assay time
- Provides full length Tankyrase 1 enzyme and unmodified NAD substrate
Kit Contents
- Histone-coated 96-stripwell Microplate
- Anti-PAR Monoclonal Antibody
- HRP-conjugated Goat Anti-Mouse Antibody
- Tankyrase 1
- XAV939a
- Diluents & Buffers
- Unmodified NAD Substrate
- HRP Substrate
Data Example
Inhibition of Tankyrase 1 Activity by XAV939
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| Graphical representation of a chemiluminescent Tankyrase 1 standard curve (A) and an inhibition curve for the Tankyrase 1 inhibitor XAV939 (provided in the kit; B). The standard curve is used to translate absorbance values to activity units of Tankyrase 1. |
aXAV939 is a Tankyrase 1 inhibitor